Center for Membrane Protein Research
Mission: The long-term goal of the Center is to advance our knowledge of the structure and function of membrane proteins in health and disease. The Center brings together a group of TTUHSC and TTU investigators interested in the broad field of membrane-protein research.
Rationale: After completion of the human genome sequence, biomedical research has evolved into a combination of genomics, proteomics, and functional genomics. To a great extent, biomedical research in this century will be focused on prototypical proteins and protein families, including the determination of their structures, normal function, and their roles in human disease. From this knowledge will emanate rational design of new pharmacological agents that will open novel therapeutic approaches.
The Membrane Protein Laboratory Core (MPLC), a shared facility of the Center for Membrane Protein Research (CMPR), houses equipment that is primarily accessible to the CMPR faculty and their laboratory personnel, but is also available to Faculty throughout TTUHSC and TTU. The MPLC is equipped for the overexpression, purification and characterization of membrane proteins. Some of the equipment available includes digital gel-imaging systems (UV, visible and infrared), microscopes, analytical spectrophotometer, fluorescence/absorbance microplate reader, microplate washer, luminometer, spectrofluorometer, refrigerated shaker incubators, probe sonicator, small and large volume microfluidizers, FPLC systems, phosphorescence lifetime spectrometers for luminescence resonance energy transfer measurements, rapid-mixing stop-flow system for absorbance, fluorescence and light-scattering determinations, centrifuges and ultracentrifuges, crystallization incubators and mosquito robot, automatic UV microscopy system for crystal analysis, Rigaku ScreenMachine for X-ray crystal screening and data collection, high-throughput automatic frog-oocyte injector, automatic patch-clamp system, liquid scintillation and gamma counters, isothermal titration microcalorimeter, and an electron paramagnetic resonance spectrometer.
Last revised: September 24, 2012