Research: Center of Excellence for Infectious Diseases
Haoquan Wu, Ph.D.
Assistant Professor of the Center of Excellence for Infectious Diseases
Department of Biomedical Sciences
miRNAs are a class of small RNAs around 22 nucleotides long, that have emerged as critical regulators that modulate almost all biological processes involving gene expression. Alterations in miRNA expression have been implicated in various human diseases including cancer. The major interest of the Wu lab is to understand how miRNAs are generated and degraded, and also how these processes are regulated during T cell differentiation and virus infection. And also our previous studies suggest that the sequence of a given miRNA is not fixed as annotated in the miRBase, but show considerable variations at the ends. We are assessing the functional significance of the variant miRNA forms and the mechanism of their generation.
Dr. Wu obtained his Ph.D. degree from Fudan University, Shanghai, China and received postdoctoral training at UCLA and the Immune Disease Institute, Harvard Medical School.
- Ongoing Projects
Selected Recent publications
1. Hongming Ma, Yonggan Wu, Jiang-Gi Choi, Haoquan Wu*. The Lower and Upper stem-ssRNA Junctions Together Determine the Drosha Cleavage Site. Proc Natl Acad Sci U S A. In press. *Corresponding author.
2. Chunting Ye, Jiang-Gi Choi, Sojan Abraham, Haoquan Wu, Diaz D, Terreros D, Premlata Shankar, Manjunath N. Human macrophage and dendritic cell-specific silencing of high-mobility group protein B1 ameliorates sepsis in a humanized mouse model. Proc Natl Acad Sci U S A. 2012 Dec 18;109(51):21052-7. doi: 10.1073/pnas.1216195109. Epub 2012 Dec 3.
3. Haoquan Wu*, Hongming Ma, Chunting Ye, Danielle Ramirez, Shuiping Chen, Jessica Montoya, Premlata Shankar, Xiaozhong Wang, Manjunath N. Improved siRNA/shRNA functionality by mismatched duplex. PLoS One. 2011;6(12):e28580. doi: 10.1371/journal.pone.0028580. Epub 2011 Dec 9. *Co-corresponding author.
4. Chunting Ye, Sojan Abraham, Haoquan Wu, Premlata Shankar, Manjunath N. Silencing early viral replication in macrophages and dendritic cells effectively suppresses flavivirus encephalitis.PLoS One. 2011 Mar 15;6(3):e17889. doi: 10.1371/journal.pone.0017889.
5. Haoquan Wu*, Joel R. Neilson, N. Manjunath. microRNA cloning from cells of the immune system. Methods Mol Biol. 2010;667:67-77. doi: 10.1007/978-1-60761-811-9_5. *Corresponding author.
6. Haoquan Wu*, Chunting Ye, Danielle Ramirez, N. Manjunath. Alternative Processing of Primary microRNA Transcripts by Drosha Generates 5’ End Variation of Mature microRNA. PLoS ONE 2009, 4(10): e7566. doi:10.1371/journal.pone.0007566. *Co-corresponding author.
7. N. Manjunath, Haoquan Wu, Premlata Shankar. Lentiviral delivery of short hairpin RNAs. Adv Drug Deliv Rev. 2009 Jul 25;61(9):732-45. Epub 2009 Mar 31.
8. Priti Kumar, Hong-Seok Ban, Sang-Soo Kim,Haoquan Wu, Todd Pearson, Dale Greiner, Amale Laouar, Jiahong Yao, Viraga Haridas, Katsuyoshi Habiro, Yong-Guang Yang, et al, T Cell-Specific siRNA Delivery Suppresses HIV-1 Infection in Humanized Mice. Cell. 2008 Aug 22;134(4):577-86. Epub 2008 Aug 7.
9. Haoquan Wu, Joel R. Neilson, Priti Kumar, Monika Manocha, Premlata Shankar, Phillip A. Sharp, N. Manjunath. miRNA Profiling of Naïve, Effector and Memory CD8 T Cells. PLoS ONE. 2007 Oct 10;2(10):e1020.
10. Priti Kumar, Haoquan Wu, Jodi L. McBride, Kyeong-Eun Jung, Moon Hee Kim, Beverly L. Davidson, Sang Kyung Lee, Premlata Shankar & N. Manjunath. Transvascular delivery of small interfering RNA to the central nervous system. Nature. 2007 Jul 5; 448(7149):39-43. Epub 2007 Jun 17.
Hongming Ma, Ph.D.
Post Doctoral Research Associate