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Microscope Multiphoton Imaging Analysis

Multiphoton Microscope

In live cells and tissue imaging, a key technology is two photon laser scanning microscopy (TPLSM). It minimizes phototoxic effects and offers the possibility to image deep into tissues. A set of instruments using this powerful technology is available in the Imaging Facility at School of Pharmacy.

Key Features

Simultaneous excitation imaging with two-wavelength IR laser

Deep in vivo imaging with super high-sensitive GaAsP NDD

Ultrahigh-speed imaging of up to 420 frames per second
Multi-color in vivo imaging

Deep specimen imaging with non-descanned detectors (NDD)

Nikon's high-NA objectives are ideal for multiphoton imaging

Auto laser alignment when changing multiphoton excitation wavelength

Two types of scanning head enable high-speed, high-quality imaging

NIS-Elements C acquisition and analysis software

NIKON-MICROSCOPE

Visualization of brain vessels in stroke:  3D reconstruction of mouse brain vessel. Endothelial cells stained with anti-CD31 antibody (red) and cell nuclei with Hoechst (blue).  

A 3-dimensional image showing brain micrometastases. (Green)  cells transfected with eGFP- coopting vasculature (Red)  within brain.

Endothelial Cell
3-dimensional image

Image of retina plexus of adult mouse. Whole mount retina staining. Endothelial cells stained with Alexa488-conjugated Isolectin-B4; Cell nuclei stained with Hoechst.

Co-staining of endothelial cells and pericytes in the main vessels of adult retinas. Whole mount retina staining. Endothelial cells stained with Isolectin-B4 (green) and pericytes stained with anti-NG2 Chondroitin Sulfate Proteoglycan antibody (red).

Retina Plexus Retina Endothelial Cell

Location:

Faculty Contact:

Texas Tech University Health Sciences Center- SOP
Amarillo Research Building Room 1207D
1406 Coulter St.
Amarillo, TX 79106
Map to Location

Dr. Sanjay K. Srivastava, Ph.D.

Office: ARB 2101

Phone: 806-414-9211

Email: sanjay.srivastava@ttuhsc.edu

School of Pharmacy Fees:

Staff Contact: for training and assistance

Hourly user fee: $20

Hourly rate for technician: $40

Hourly rate for others

Training Video

Online Reservation

Dr. Zijuan Liu, Ph.D.

Office: ARB 2100A

Phone: 806-414-9199

Email: zijuan.liu@ttuhsc.edu

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