TTUHSC Image Analysis Core Facility (Lubbock Campus) - The newly created Imaging Facility (1,200 square feet) has recently purchased the IVIS Lumina XR from Caliper Life Sciences for real time tracking of cells expressing bioluminescent or fluorescent proteins simultaneously with X-ray digital imaging technology for living mice. We also have in that facility two fully equipped laser scanning confocal microscopes. One of these confocal microscopes is a newly acquired Nikon Ti-E microscope with A1 confocal and STORM superresolution. The other is a Zeiss LSM 510/Meta confocal system attached to both inverted and up-right microscopes. The Nikon system is equipped to perform spectral imaging experiments, TIRF and FRAP. A high temporal and spatial resolution EMCCD camera is attached to this system. The microscope is also equipped with a stage-top environmental chamber in which precision temperature incubations can be performed. In addition, we also have up-right and inverted fluorescence microscopes attached to a CCD camera for routine observation of stained (fluorescence) or unstained (Differential Interference Contrast, DIC) specimens. Link: Image Analysis Core Facility
TTUHSC Molecular Biology Core Facility - Mission: The TTUHSC Molecular Biology Core Facility provides state-of-the-art instrumentation to support experimental demands in molecular biology, ranging from routine bacterial cell growth, monitoring of mammalian cell growth, advanced approaches for cell transfection, monitoring protein and gene expression, RT-PCR, flow cytometer and cell sorting capabilities, exon amplification and single cell separation for whole genomic analysis. Expertise and advice is available for the design of experiments using these approaches and technologies.
Services and Available Equipment: The Molecular Biology Core Facility has a Meso Scale Discover Sector Imager that uses multi-array approaches enabling the detection of bio-markers in single and multiplex formats. For quantification and analysis of DNA, RNA and proteins we have the ImageQuant LAS 4000 digital imager and the Typhoon FLA 9000 Biomolecular Imager for quantitative imaging of chemiluminescence, fluorescence, and gel documentation. The 2100 Bioanalyzer (Agilent) it uses microfluidics to run DNA, RNA and proteins samples and replaces agarose gels and SDS-PAGE. The Infinite M1000 PRO quadruple monochomator microplate reader (Tecan) is used to measure UV, VIS absorption, and a variety of fluorescence-based approaches with high spectral resolution. For transfection of cells with DNA or RNA, we have the 4D-Nucleofector Core X/Y (Lonza). For cell growth (mammalian cells in suspension, bacteria and yeast) we have the Biolector MP2 that allows continuous monitoring of growth mass, fluorescence, acid production and oxygen consumption in multi-well format. For analysis of gene expression, micro RNA profiling and non-coding RNA analysis we have the Quant Studio 12K real time PCR (Life Technologies). High throughput target enrichment (exons) and amplicon tagging is performed with Access Array (Fluidigm). The C1 Single Cell auto preparation system (Fluidigm) allows us to study in single cells gene expression and mRNA analysis in a 96 well format. A very simple image cytometric analysis to study apoptosis, cell cycle analysis, cell proliferation, viability assays and cell counting is performed using the Cellometer from Vision Image Cytometry. High resolution microscopy in flow (ImageStream MarKK II, Amnis) is used to evaluate microscopically the localization of subcellular proteins with a high throughput approach using fluorescently labeled samples. The BD FACSJazz cell sorter and the BD Accuri C6 flow cytometer allows to perform cell sorting and study many cellular functions including apoptosis, cell cycle, cell proliferation, cell phenotyping. Click here for photos of equipment. Click here for description of equipment.
To see other items of shared equipment at the TTUHSC please click here.
To go to the Image Analysis Core Facility please click here.
Contact Information: Dr. Sennoune is the director of the Molecular Biology Core Facility and is associated with the Department of Cell Physiology and Molecular Biophysics. CVs (Sennoune) - phone (806)743-2535. Molecular Biology Core Facility phone (806)743-4624
Center for Biotechnology and Genomics (TTU General Academic Lubbock Campus)- Provides instrumentation, support and training in various aspects of modern biotechnology including spectrophotometry, protein identification, protein purification molecular interaction, DNA applications an functional genomics. Link: http://www.depts.ttu.edu/biotechnologyandgenomics/
Membrane Protein Core Facility (Lubbock HSC Campus) - The Membrane Protein Laboratory Core (MPLC), is a shared facility located in the Center for Membrane Protein Research (CMPR), houses equipment that is primarily accessible to the CMPR faculty and their laboratory personnel, but is also available to Faculty throughout TTUHSC and TTU. The MPLC is equipped for the overexpression, purification and characterization of membrane proteins. Some of the equipment available includes digital gel-imaging systems (UV, visible and infrared), microscopes, spectrophotometers, a fluorescence/absorbance microplate reader, a microplate washer, a luminometer, a spectrofluorometer, refrigerated shaker incubators, a probe sonicator, small and large volume microfluidizers, FPLC systems, phosphorescence lifetime spectrometers for luminescence resonance energy transfer measurements, rapid-mixing stop-flow system for absorbance, fluorescence and light-scattering determinations, ATR/FTIR spectrometer for secondary structure determination, a picosecond lifetime system, static, and dynamic light scattering instruments, centrifuges and ultracentrifuges, crystallization incubators and mosquito robot, automatic UV microscopy system for crystal analysis, Rigaku Screen Machine for X-ray crystal screening and data collection, one- and two-photon confocal microscopy systems, high-throughput automatic frog-oocyte injector, liquid scintillation and gamma counters and an electron paramagnetic resonance spectrometer. Link: www.ttuhsc.edu/cmpr/mpcl.aspx
Neuroimaging Core (TTU General Academic Lubbock Campus) - The Texas Tech Neuroimaging Institute (TTNI) is a multi-user neuroimaging facility that promotes cutting-edge interdisciplinary research among Texas Tech University and Texas Tech University Health Sciences Center faculty and graduate students. The TTNI provides researchers with brain and body imaging technologies including structural (MRI), functional magnetic resonance imaging (fMRI), magnetic resonance spectroscopy, diffusion tensor imaging and techniques, including multimodal data fusion of EEG, fMRI, and DTI data. Link - http://www.depts.ttu.edu/vpr/ttni/
Digestive Disease and Molecular Medicine Core (TTUHSC El Paso Campus) - Various clinical investigative testings are available including: Electrogastrogram (EGG); SmartPill, i.e. Wireless GI Motility Testing System; Interrogation Programming for gastric neurostimulation therapy (Enterra Device); autonomic testing and Transcutaneous Electrical Acupuncture System all of which are highly applicable for diagnostic and therapeutic intervention in GI tract motility disorders. The core is also capable of testing for genes and gene expression, gene SNP in health & disease, analyzing for cytokines and growth factors in serum as well as all collectible secretions and excretions with emphasis on promotion of translational research that may potentially lead to discovery of new biomarkers or therapeutic remedies for internal medicine. Laboratory Instrumentation available includes: Real-Time PCR System ViiA 7; Ultracentrifuge; Cone/Plate Viscometer; ELISA Plate Reader; Cell Culture CO2 Incubator; UV/VIS Spectrophotometer; and Biological Safety Level 2 Laminar Flow Cabinets. Contact: Jerzy.Sarosiek@ttuhsc.edu
Department of Medical Photography and Electron Microscopy supports all faculty, staff and students of Texas Tech University Health Sciences Center. We specialize in producing a wide array of imaging services for a variety of needs including instructional use, research, student assignments or official business. We are well known for our award winning photography, high quality research posters, graphic design and specialized transmission and scanning electron microscopic work. We are located in Room BC200 of the Health Sciences Center. Please call (806) 743-1366 for additional information. Link: http://www.ttuhsc.edu/som/medicalphotography/
TTU Imaging Center - The TTU Imaging Center is located in the Biology Department at the Lubbock TTU campus. They can prepare samples for both light and electron microscopy.
Cancer Center Cores: The TTUHSC SOM Cancer Center has several core laboratories that are also available to non-cancer faculty. It is located on the TTUHSC Lubbock Campus.
- Cell Culture Core, which serves as the Cell Culture and Xenograft Repository for the Children’s Oncology Group (www.COGcell.org) and as the Texas Cancer Cell Repository (www.TXCCR.org).
- Cell Identification Core, which carries out short tandem repeat analyses of cell lines and compares them to a > 3000 cell line database to verify identity.
- Flow Cytometry Core, which has a BD LSR-II analyzer and a BD FACSaria cell sorter.
- Immunocompromised Mouse Core, which is part of the TTUHSC LARC and has laminar flow caging systems, animal irradiator, and anesthesia.
- Molecular Biology Core, which has a Taqman quantitative RT-PCR (TLDA-capable), and Agilent bioanalyzer, and an ABI sequencer optimized for STR analysis.
Further information, including contact information, can be found at: http://cancer.ttuhsc.edu/core/