TTUHSC Image Analysis Core Facility
The TTUHSC Imaging Center provides state-of-the-art instrumentation for high-resolution
light microscopic imaging. Expertise and advice is available for the design of experiments
involving these techniques; and for the development of grants and manuscripts involving
high-resolution light microscopic technologies.
Services and Available Equipment
The imaging facility has a IVIS Lumina XR from the Caliper Life Sciences for real
time tracking of cells expressing bioluminescent or fluorescent proteins simultaneously
with X-ray digital imaging technology for living mice. We also have two fully equipped
laser scanning confocal microscopes. One of these confocal microscopes is a newly
acquired Nikon Ti-E microscope with A1 confocal and STORM super resolution. The other
is a Zeiss LSM 510/Meta confocal system attached to both inverted and upright microscopes.
The Nikon system is equipped to perform spectral imaging experiments, TIRF and FRAP.
A high temporal and spatial resolution EMCCD camera is attached to this system. The
microscope is also equipped with a stage-top environmental chamber in which precision
temperature incubations can be performed. In addition, we also have upright and inverted
fluorescence microscopes attached to a CCD camera for routine observation of stained
(fluorescence) or unstained (Differential Interference Contrast, DIC) specimens.
Click here for photos of equipment. Click here for description of equipment.
If you are a TTUHSC user, please click here to see the Image Analysis Core Laboratory Rates for Internal (TTUHSC) users. If you are a Non-TTUHSC user, please click here to see the Core Imaging Facility Rates for External (Non-TTUHSC) users.
To see other items of shared equipment at the TTUHSC please click here.
Dr. Grozdanov is the managing director of the TTUHSC Core Laboratories and is associated
with the Department of Cell Biology and Biochemistry.
Email - firstname.lastname@example.org
Image Analysis Core Facility phone - (806)743-4624
Image Analysis Core Facility Policies
Training for all systems is mandatory before they can be used independently. Contact
the Image Analysis Core Facility Managing Director to schedule a time. In your email,
please provide a brief description of what you would like to image (e.g., fixed tissue,
fixed cells, live cells, etc.), and the fluorophores you are planning to use. The
amount of training required depends on the system, the user’s background, and the
samples. For a new user, the training usually takes between 1.5 to 3 hours.
- Microscope sessions must be scheduled through the SharePoint Microscope reservation webpage. Instruction on how to make the reservation can be found here. You have to email the Image Analysis Core Facility Managing Director to be granted
access to the SharePoint Microscope Scheduling system.
- Be realistic with your scheduling.
- It is your responsibility to know if someone has booked the microscope after you.
You should check the reservation webpage about 30 minutes before the start of your
session. If you are using a microscope and the next user is coming less than 2 hours
after your session ends, leave everything on—this will prolong the life of the mercury
lamps and the health of the lasers.
- If you add a new reservation for a microscope while another user is on that microscope,
or less than 30 minutes before the start of that user’s session, it is your responsibility
to tell that user and to ask her or him to leave the microscope on for you.
- If you receive instrument specific instructions about this during training, those
instructions supersede the information in this section.
DATA TRANSFER and DATA STORAGE
- If you need to use the computer connected to a microscope for data transfer to a portable
hard drive, please reserve it as you would normally. This is the only way to ensure
its availability. There will not be a charge for a data transfer.
- You should consider doing the data transfer while you are imaging to maximize the
availability of the microscope.
- Users are encouraged to back up files frequently to reduce data transfer time.
- The Image Analysis Core Facility assumes NO responsibility for data storage. All hard
drives will eventually fail. It is impossible to protect data from viruses with 100%
confidence. It is the user’s responsibility to backup data.
Image Analysis Core Facility should be listed in the “Acknowledgments” section of
any publication using data generated in the Image Analysis Core Facility as follows:
“Images were generated in the Image Analysis Core Facility supported in part by TTUHSC.”
Proper acknowledgment will show that the facility is used, and to enable growth and
continuation of financial and other support, so that it may continue to provide its
essential services in the best way possible.