TTUHSC Research
HomeResearch

Molecular Biology Core Facilities Equipment Photos


To use this equipment, contact Dr. Grozdanov at molcore@ttuhsc.edu
 or phone: +1 (806) 743-4624


2100BA
2100 Bioanalyzer (Agilent). The instrument uses microfluidics to resolve RNA, DNA, and protein samples. It is useful to determine the quality of RNA (RNA integrity number) before running microarrays, RNA-seq or real time quantitative PCR measurements. The individual samples are analyzed and a pseudogel image is created. The nucleic acid or protein bands are sized and quantified. The instrument requires minimal amount of sample (4µl). It replaces time-consuming techniques associated with agarose gels or SDS-PAGE with fast, automated, high quality digital data. Preparation time is 5 min and digital data is obtained in 30 minutes.
More information about the instrument is available on the company's webpage.

BMP2
Biolector (MP2 labs). The instrument can be used to grow bacteria, yeast, or mammalian cells in suspension in a 48-well plate. Cell growth (biomass), fluorescence, acid production (pH) and oxygen consumption can be continuously and simultaneously monitored. CO2, oxygen, humidity and temperature are also fully controlled and adjusted. The instrument allows rapid optimization of growth parameters and implementation of cytotoxicity bioassays. A companion for this system is a shaker (Multitron II; offline) that allows performing parallelized experiments, thus freeing the time in the Biolector.
More information about the instrument is available on the company's webpage

CVIC
Cellometer Vision Image Cytometry (Nexcelom Bioscience). The instrument can be used for simple image cytometry in a 20 µl sample, capable of performing cell cycle analysis, apoptosis, autophagy, cell proliferation, mitochondrial membrane potential, viability assays, morphology analysis and cell counting. Equipped to monitor fluorescence of GFP, FITC, Acridine Orange + DNA, Acridine Orange + RNA, RFP, PI, PE, as well as other commonly used fluorochromes in green and red spectra. It captures images using an integrated digital camera and 20x objective.
More information about the instrument can be obtained on the company's webpage

AAF
Access Array System (Fluidigm). The access array is a high throughput target-enrichment system. It can enrich multiple unique targets (e.g. exons) from a large number of samples, all at the same time. Multiple samples and amplicons can be simultaneously barcoded (indexed), which streamlines next generation sequencing library preparation.
More information about the instrument is available on the company's webpage

 C1SAPP
C1 Single-Cell Auto Prep System (Fluidigm). Useful to study single-cell gene expression, single-cell mRNA analysis, and single-cell genomics. It uses microfluidic technology that allows rapid and reliable isolation, processing, and profiling of 96 individual cells. The use of C1 Single-Cell Auto Prep System can be combined with the Access Array System (see above).
More information about the C1 Single-Cell Auto Prep System is available on the company's webpage.

 BDAC6FC
BD Accuri C6 flow cytometer (BD Biosciences). The system is equipped with a blue and red laser, and four fluorescence detectors with optical filters optimized for the detection of most common fluorophores (e.g., FITC, PE, PerCP, and APC). Any brand of 12 x 75 mm sample tubes and/ or microcentrifuge tubes can be used for the sample preparation. The instrument is also capable of automated data collection from 48- and 96-well plates. It can be used to study many cellular functions, including apoptosis, cell cycle, cell proliferation, mitochondrial membrane potential, immunoassays, cell phenotyping, cytosolic calcium and pH, nitric oxide.
More information about the instrument is available on the company's webpage.

 HRM
ImageStream Mark II (Amnis). This system combines the speed, statistical power, and fluorescence sensitivity of flow cytometry with the functional insights (e.g. morphology, fluorescent localization) of high-resolution microscopy, making it a high-resolution microscopy in flow. It is equipped with 20x, 40x and 60x objectives, 4 lasers (405 nm, 488 nm, 561 nm, 642 nm, 785 nm), and 12 imaging channels. Dual CCD cameras are used as detectors. This system can be used to evaluate many different fluorochromes in non-adherent cells, i.e., cells in suspension alive or fixed.
More information about the ImageStream Mark II is available on the company's webpage.

 BDFACSJ
BD FACSJazz cell sorter (BD Biosciences). The instrument is equipped with three lasers [violet (405 nm), blue (488 nm) and red (640 nm)] and six filters. This configuration allows the use of many typical fluorophores (e.g., FITC, GFP, PE, PI PerCP-Cy 5.5, PE-Cy7, APC, APC-H7, CFP, DAPI). The sheath pressure and drop drive frequency are pre-selected to minimize set-up of sorting parameters. Useful for immunophenotyping, stem cell and cancer research, among many other applications. It is housed in a biological safety cabinet with high efficiency particulate air (HEPA) filters, to prevent accidental exposure of the operators to biological samples.
More information about the FACSJazz instrument is available on the company's webpage.

MSDSI2400
SECTOR Imager 2400 (Meso Scale Discover, MSD). This multi-array technology (96- and 384-well, small spot) enables the detection of biomarkers in single and multiplex formats. It utilizes special kits for profiling biomarkers, cell signaling pathways, cancer markers, angiogenesis, cardiovascular diseases, neurodegenerative disorders, apoptosis, metabolites, cytokines and others. It can also be customized for individual applications. It is equipped with an ultra-low noise CCD camera for high dynamic range, sensitivity and rapid read times.
For more information about the Sector Imager visit the company's webpage.

IM1000
Infinite M100 PRO Quadruple monochromator microplate reader (Tecan). Automated microplate reader for UV, VIS absorption, fluorescence intensity, and fluorescence polarization with time resolved fluorescence, fluorescence resonance energy transfer and alpha screen technology. It can also quantify luminescence and luminescence spectra. Temperature controlled system (up to 42°C) and orbital and linear shaking capabilities. Accommodates 6-, 12-, 24-, 48-, 96-, 384-, and 1536-well plate formats. The spectral resolution is 1 nm.
More information about the Infinite M100 PRO is available on the company's webpage.

IM4000
ImageQuant LAS 4000 (GE Healthcare Life Sciences).  The instrument is a multipurpose 16-bit 3.2 megapixel CCD camera system for sensitive and quantitative imaging of chemiluminescence, standard UV transillumination, or white light gel or blot documentation.
More information about this instrument can be accessed on the company’s webpage.
QS12KFlex 
Quant Studio 12K Flex real-time PCR system (Life Technologies).  The instrument can be used for gene expression analysis, microRNA profiling, and non-coding RNA analysis using TaqMan or SYBR Green assays. It supports real-time PCR in 96- and 384-well plate formats as well as TaqMan Low density Array cards.
For more information click here.
TyphoonFLA9000 
The Typhoon FLA 9000 Biomolecular Imager is a variable mode laser scanner for flexible, sensitive imaging and quantitation of proteins, nucleic acids, and other molecules using filmless autoradiography, fluorescence, chemifluorescence, and gel documentation.
For more information please click here.

To use this equipment, contact Dr. Grozdanov at molcore@ttuhsc.edu
 or phone: +1 (806) 743-4624

©