Pharmacology and Neuroscience
Dr. Dean Smith
Vice President - Research
During the past four years, we have been culturing embryonic stem cells (rodent and
human) with the goal of directing their differentiation into neurons. In that context,
we have been characterizing various physiological features at different stages of
stem-cell differentiation, with special emphasis on voltage-gated potassium channels.
We use electrophysiological (mainly patch-clamp), immunocytochemical, and biochemical
(for example, Western blot and PCR) assay procedures.The goal is to develop highly-purified
populations of neurons with predictable physiological characteristics that are suitable
for therapeutic use.
Hamilton, B.R. and Smith, D.O. (1991). Autoreceptor-mediated purinergic and cholinergic
inhibition of motor nerve terminal calcium currents in rats. J. Physiol. (Lond.) 432,
Lu, Z. and Smith, D.O. (1991). Adenosine 5'-triphosphate increases acetylcholine channel
opening frequency in rat skeletal muscle.J. Physiol. (Lond.), 436, 45-56.
Smith, D.O., Franke, C., Rosenheimer, J.L., Zufall, F., and Hatt, H. (1991). Glutamate-activated
channels in adult rat ventral spinal cord cells. J. Neurophysiol., 66, 369-378.
Smith, D.O., Franke, C., Rosenheimer, J.L., Zufall, F., and Hatt, H. (1991). Desensitization
and resensitization rates of glutamate-activated channels may regulate motoneuron
excitability. J. Neurophysiol., 60, 1166-1175.
Smith, D.O., Conklin, M.W., Jensen, P.J., and Atchison, W.D. (1995). Decreased calcium
currents in motor nerve terminals of mice with Lambert-Eaton myasthenic syndrome.
J. Physiol. (Lond). 487.1, 115-123.
Smith, D.O., Jensen, P., Lowe, D., Temkin, R., and Hatt, H. (1995). Dopamine increases
glutamate-activated currents in spinal motoneurons. J. Neurosci. 15, 3905-3912.
Cui, C., Smith, D.O., and Adler, J. (1995). Characterization of mechanosensitive channels
in Escherichia coli cytoplasmic membrane by whole-cell patch clamp recording.J. Memb.
Biol. 144, 31 42.
Good, T.A., Smith, D.O., and Murphy, R.M. (1996). β-amyloid peptide blocks the fast-inactivating
K+current in rat hippocampal neurons. Biophys. J. 70, 296-304.
Lowe, D.L., Jahn, K., and Smith, D.O. (1997). Glutamate receptor editing in the mammalian
hippocampus and avian neurons. Mol. Brain Res. 48, 37-44.
Temkin, R., Lowe, D.L., Jensen, P., Hatt, H., and Smith, D.O. (1997). Expression of
glutamate receptor subunits in α-motoneurons. Mol. Brain Res., 52, 38-45.
For further information contact Dr.Dean Smith