Cell Physiology and Molecular Biophysics
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Lan Guan, M.D., Ph.D.Assistant ProfessorCell Physiology and Molecular BiophysicsPh.D.,Tokai University School of Medicine,Isehara Japan, 2000 |
Texas Tech University Health Sciences Center
3601 4th Street, STOP 6551
Lubbock, Texas 79430
Phone: (806) 743-3102
FAX: (806) 743-1512
Email: Lan.Guan@ttuhsc.edu
Research Interests
Membrane transport proteins catalyze translocation of solutes across cell membranes, and play crucial roles in many aspects of cellular functions. Many membrane proteins are targets for pharmacologically and toxicologically active substances. My laboratory focuses on the crystal structure determination and the mechanism of cation-coupled symporters. We are developing novel capture reagents to facilitate the structure determination.
Current Publication list:
Research Articles
[25] Chaptal, V, Kwon, S, Sawaya, MR, Guan, L, Kaback, HR, and Abramson, J (2011). Crystal structure of lactose permease in complex with an affinity inactivator yields new insight into sugar recognition. Proc Natl Acad Sci U S A. 108(23):9361-6. PMID: 21593407
[24] Kudugunti, SK, Thorsheim, H, Yousef, MS, Guan, L, and Moridani, MY (2011). The metabolic bioactivation of caffeic acid phenethyl ester mediated by tyrosinase selectively inhibits glutathione S-transferase. Chem Biol Interact. 192(3):243-56. PMID: 21458432
[23] Guan, L., Nurva, S., and Ankeshwarapu, S. P. (2011) Mechanism of melibiose/cation symport of the melibiose permease of Salmonella typhimurium. J Biol Chem. 286:6367-74 PMID: 21148559
[22] Chae, P. S., Rasmussen S. G. F., Rana, R., Gotfryd, K., Chandra, R., Goren, M. A., Kruse, A. C., Nurva, S., Loland, C. C., Pierre, Y., Drew, D., Popot, J. L., Picot, D., Fox, B. G., Guan, L., Gether, U., Byrne, B., Kobilka, B., and Gellman, S. H. (2010). Maltose–neopentyl glycol (MNG) amphiphiles for solubilization, stabilization, and crystallization of membrane proteins. Nature Methods 7(12):1003-8. PMID: 21037590
[21] Guan, L. and Kaback, H. R. (2009). Properties of a LacY efflux mutant. Biochemistry 48(39):9250-5. PMCID: PMC2754601
[20] Yousef, M. S. & Guan, L. (2009). A 3-D structure model of the melibiose permease of Escherichia coli represents a distinctive fold for Na+ symporters. Proc Natl Acad Sci U S A. 106:15291-6. PMCID: PMC2729278
[19] Zhou, Y. G., Guan, L., Freites, J. A., and Kaback, H. R. (2008). Opening and closing of the periplasmic gate in lactose permease. Proc Natl Acad Sci U S A, 105 (10):3774-3778. PMCID: PMC2268789
[18] Guan, L., Mirza, O., Verner, G., Iwata, S, and Kaback, H. R. (2007) Structural determination of the wild-type lactose permease. Proc Natl Acad Sci U S A, 104 (39):15294-8. PMCID: PMC2000551
[17] Guan, L. & Kaback, H. R. (2007). Site-directed alkylation of cysteine to test solvent accessibility of membrane proteins. Nature protocol, 2, 2012 -7. PMCID: PMC2775809
[16] Orsolya, K.*, Guan, L.*, Szepessy, E., Kukor, Z., Tóth, M., and Sahin-Tóth, M. (2006). Expression of human cationic trypsinogen with an authentic N terminus using inteinmediated splicing in aminopeptidase P (pepP) deficient Escherichia coli. Protein Expr Purif., 48(1):104-11 (*equal contribution). PMCID: PMC1604731
[15] Mirza, O.*, Guan, L.*, Verner, G., Iwata, S., and Kaback, H. R. (2006). Structural evidence for sugar recognition and mechanism for galactoside/H+ symport in the lactose permease. EMBO J, 25:1177-83 (*equal contribution). PMCID: PMC1422171
[14] Guan, L., Smirnova, I., Verner, G., Nagamoni, S., and Kaback, H. R. (2006). Manipulating phospholipids for crystallization of a membrane transport protein. Proc Natl Acad Sci U S A, 103 (6):1723-1726. PMCID: PMC1413674
[13] Guan, L. and Kaback, H. R. (2004). Binding affinity of lactose permease is not altered by the H+ electrochemical gradient. Proc Natl Acad Sci U S A, 101 (33):12148-52. PMCID: PMC514448
[12] Vázquez-Ibar, J. L., Guan, L., Weinglass, A. B., Verner, G., Gordillo, R., and Kaback, H. R. (2004). Sugar recognition by the lactose permease of Escherichia coli. J Biol Chem, 19;279(47):49214-21. PMID: 15364943
[11] Vázquez-Ibar, J. L.*, Guan, L.*, Svrakic, M., and Kaback, H. R. (2003). Exploiting luminescence spectroscopy to elucidate the interaction between sugar and a tryptophan residue in the lactose permease of Escherichia coli. Proc Natl Acad Sci U S A, 100 (22):12706-11 (*equal contribution). PMCID: PMC240682
[10] Ermolova, N., Guan, L., and Kaback, H. R. (2003). Intermolecular thiol cross-linking via loops in the lactose permease of Escherichia coli. Submitted to Proc Natl Acad Sci U S A, 100 (18):10187-92. PMCID: PMC193537
[9] Guan, L., Sahin-Toth, M., Kalai, T., Hideg, K., and Kaback, H. R. (2003). Probing the mechanism of a membrane transport protein with affinity inactivators. J Biol Chem, 278(12):10541-8 (cover of JBC). PMID: 12471022
[8] Guan, L., Hu, Y.L., and Kaback, H. R. (2003). Aromatic stacking in sugar binding site of lactose permease in Escherichia coli. Biochemistry, 42(6):1377-82, (accelerated publication). PMID: 12578349
[7] Sorgen, P. L., Hu, Y. L., Guan, L., Kaback, R. H., and Girvin, M. (2002). An approach to membrane protein structure without crystals. Proc Natl Acad Sci U S A, 99(22):14037-40. PMCID: PMC137832
[6] Guan, L., Sahin-Toth, M., and Kaback, H. R. (2002). Changing the lactose permease of Escherichia coli into a galactose-specific symporter. Proc Natl Acad Sci U S A, 99(10):6613-8. PMCID: PMC124451
[5] Guan, L., Murphy, F. D., and Kaback, H. R. (2002). Surface-exposed positions in the transmembrane helices of the lactose permease of Escherichia coli determined by intermolecular thiol cross-linking. Proc Natl Acad Sci U S A, 99(6):3475-80. PMCID: PMC122548
[4] Zhang, W., Guan, L., and Kaback, H. R. (2002). Helices VII and X in the lactose permease of Escherichia coli: proximity and ligand-induced distance changes. J Mol Biol, 315(1):53-62. PMID: 11771965
[3] Guan, L., Weinglass, A. B., and Kaback, H. R. (2001). Helix packing in the lactose permease of Escherichia coli: localization of helix VI. J Mol Biol, 312(1):69-77. 11545586
[2] Guan, L. and Nakae, T. (2001). Identification of essential charged residues in transmembrane segments of the multidrug transporter MexB of Pseudomonas aeruginosa. J Bacteriol, 183(5):1734-9. PMCID: PMC95059
[1] Guan, L., Ehrmann, M., Yoneyama, H., and Nakae, T. (1999). Membrane topology of the xenobiotic-exporting subunit, MexB, of the MexA,B-OprM extrusion pump in Pseudomonas aeruginosa. J Biol Chem, 4(15):10517-22. PMID: 10187844 >> Download PDF File
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Book Chapters and Reviews
Guan, L. & Kaback, H. R. (2011, in press). Glucose/Sugar Transport in Bacteria. Encyclopedia of Biological Chemistry, 2nd edition, Elsevier Inc.
Kiraly, O., Guan, L., and Sahin-Toth, M. (2011) Expression of recombinant proteins with uniform N-termini. Heterologous Gen Expression in E. coli, Methods in Molecular Biology. Springer Science & Business Media, LLC. Volume 705 (Chapter 10), 175-194. PMID: 21125386
Guan, L. & Kaback, H. R. (2006). Lessons from lactose permease. Annual Review of Biophysics & Biomolecular Structure (35):67-91. PMCID: PMC2802108
Guan, L. & Kaback, H. R. (2004). Glucose/Sugar Transport in Bacteria. Encyclopedia of Biological Chemistry, Volume 2. p. 204-207, Elsevier Inc.
Nakae, T., Guan, L., Yoneyama, H., Germ, M., and Nakajima, A. (2002). The topological localization of the subunit proteins of the three-component RND-efflux pump, MexAB-OprM in Pseudomonas aeruginosa. Proceedings of SPIE (4625):106-117.
Last revised, July 8, 2011