Cell Physiology and Molecular Biophysics
Jose Perez-ZoghbiAssistant Professor of Cell Physiology
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Texas Tech University Health Sciences Center
3601 4th Street, STOP 6551
Lubbock, Texas 79430
Phone: (806) 743-2522
FAX: (806) 743-1512
Email: Jose.Perez-Zoghbi@ttuhsc.edu
Research Interests
My laboratory is interested in understanding cellular mechanisms underlying the lung diseases such as asthma, COPD (Chronic Obstructive Pulmonary Disease), and pulmonary hypertension by using "living" lung slices prepared from animals in combination with molecular, pharmacological and imaging tools including confocal microscopy. Currently, we are studying the mechanisms of regulation of intrapulmonary airway and artery smooth muscle contraction. We have found that the frequency of intracellular Ca2+ oscillations and the increase in Ca2+ sensitivity, stimulated by agonists, regulate the contraction of both intrapulmonary airways and arteries. We are also exploring the effects of inflammatory mediators associated with lung pathologies on the contractility and Ca2+ signaling of airway and artery smooth muscle cells.
Link to Perez-Zoghbi Laboratory
EDUCATION
2002: Ph.D. in Biochemistry. Centro de Biofísica y Bioquímica. Instituto Venezolano de Investigaciones Científicas (IVIC). Dissertation: Kinetics of activation of gastric acid secretion in relation with [Ca2+]i changes during stimulation with secretagogues. Advisor: Dr. Fabian Michelangeli.
1996: Degree in Biology, Minor: Cell Biology. Universidad Central de Venezuela (UCV). Thesis: Changes in membrane permeability and cell death associated to rotavirus infection in cultured cells. Advisor: Dr. Fabian Michelangeli.
ACADEMIC APPOINTMENTS
2007-Present: Assistant Professor of Cell Physiology and Molecular Biophysics, Texas Tech University Health Sciences Center, Lubbock, TX.
2002-2007: Post-doctoral Associate. University of Massachusetts Medical School. PI Dr. Michael J. Sanderson.
1996-2001: Graduate Student. Centro de Estudios Avanzados (CEA). IVIC. Ph.D. in Biochemistry.
1995-1996: Research Fellow. Laboratorio de Fisiología Gastrointestinal. Centro de Biofísica y Bioquímica. IVIC.
1992-1995: Research Fellow. Biotechnology Laboratory. Facultad de Ciencias. UCV. Project: "Genetic improvement of Cereals and Legumes".
TEACHING EXPERIENCE
1993. Teaching Assistant: "Biochemistry Laboratory". Facultad de Ciencias, UCV.
1992. Teaching Assistant: "Introductory Course to Science". Facultad de Ciencias, UCV.
1991. Teaching Assistant: "Introductory Course to Science". Facultad de Ciencias, UCV.
1991. Teaching Assistant: "Biostatistics". Facultad de Ciencias, UCV.
FELLOWSHIPS
1998-2001. Investigator Promotion Program of Venezuela, PPI-3452.
1996-2000. Fellowship for Excellent Students (IVIC)
1993-1996. Student Fellowship (IVIC).
AWARDS
Paul F. Cranefield, Post-Doc Award, 2006. The Society of General Physiologists. Woods Hole, MA, USA.
National Prize to the Best Scientific Publication, 2002. Science and Technology Ministry of Venezuela. (Winner Publication: Perez et al., 2001). Venezuela.
Premio Joven Talento en Ciencias da Vida, 1999. Amersham Pharmacia Biotech. Caxambu, Brazil.
Honorific Award to Biology Degree Thesis, 1997. Facultad de Ciencias, UCV. Venezuela.
PUBLICATIONS
REFEREED ARTICLES
Perez-Zoghbi, J.F. and Sanderson, M.J. (2008). Nitric oxide induces airway smooth muscle cell relaxation by decreasing the frequency of agonist-induced Ca2+ oscillations. In revision, J Gen Physiol.
Perez-Zoghbi, J.F.; Karner, C.; Ito, S.; Shepherd, M; Alrashdan, Y.; and Sanderson, MJ (2008): Ion channel regulation of intracellular calcium and airway smooth muscle function. Pulm Pharmacol Ther, In press.
Perez-Zoghbi, J.F.; Mayora, A.; Ruiz, M-C. and Michelangeli, F. (2008): Heterogeneity of acid secretion induced by carbachol and histamine along the gastric gland axis and its relationship to [Ca2+]i. Am J Physiol Gastrointest Liver Physiol. Jul 31. [Epub ahead of print]
Sanderson, M.J.; Delmotte, P, Bai, Y. and Perez-Zogbhi, J.F. (2007): Regulation of airway SMC contractility by Ca2+ signaling and sensitivity. Proc Am Thorac Soc. 4: 1-9.
Perez-Zoghbi, JF and Sanderson, M.J. (2007): Endothelin-induced contraction of bronchiole and pulmonary arteriole smooth muscle cells is regulated by intracellular Ca2+ oscillations and Ca2+ sensitization. Am. J. Physiol. Lung. Cell. Mol. Physiol. 293: L1000-L1011.
Perez, J.F. and Sanderson, M.J. (2005): The contraction of smooth muscle cells of intrapulmonary arterioles is determined by the frequency of Ca2+ oscillations induced by 5-HT and KCl. J. Gen. Physiol. 125: 555-67
Perez, J.F. and Sanderson, M.J. (2005): The frequency of calcium oscillations induced by 5-HT, ACH, and KCl determine the contraction of smooth muscle cells of intrapulmonary bronchioles. J. Gen. Physiol. 125: 535-53
Perez, J.F.; Ruiz, M-C and Michelangeli, F. (2001): Simultaneous measurement and imaging of intracellular Ca2+ and H+ transport in isolated rabbit gastric glands. J. Physiol. 537: 735-45
Perez, J.F.; Ruiz, M-C; Chemello M-E; Michelangeli, F. (1999): Characterization of a membrane calcium pathway induced by rotavirus infection in culture cells. Journal of Virology 73: 2481-2490.
Perez, J.F.; Chemello, M.E.; Liprandi F; Ruiz, M-C.; Michelangeli, F. (1998): Oncosis in MA104 cells is induced by rotavirus infection through an increase in intracellular Ca2+ concentration. Virology 252: 17-27.
D'Suze, G.; Sevcik, C.; Perez, J.F. and Fox, J.W. (1997): Isolation and Characterization of a potent curarizing polypeptide from Tiyus discrepans scorpion venom. Toxicon 36: 1683-1689.
BOOK CHAPTERS
Perez, J.F.; Ruiz, M.C. and Michelangeli F. (2002): Measurement of intracellular calcium and gastric acid secretion in real time using fluorescent probes. In: Mechanisms and Consequences of Proton Transport. Eds Tetsuro Urushidani, John Forte and George Sachs. Kluwer (Boston), Chapter 31. pp275-278.
Michelangeli F, Perez, JF and Ruiz, M.C. (2002): Kinetics of Activation of H+ Secretion by Secretagogues in Relation to Changes in [Ca2+]i in Isolated Gastric Glands. In: Mechanisms and Consequences of Proton Transport. Eds Tetsuro Urushidani, John Forte and George Sachs. Kluwer (Boston), Chapter 30; pp265-273.