Confocal microscopy utilizes the basic principles of fluorescence microscopy, but unlike regular epifluorescence (widefield) microscopes, confocal microscopes take advantage of pinhole apertures to preferentially permit focused (or "aligned") light through each pinhole to provide a visual representation of the specimen. This feature provides enhanced contrast and superior optical sectioning capabilities; therefore, this technique is ideal for thicker specimens such as multiple cell layers or tissues.
Location: School of Pharmacy
1300 Coulter - B33
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Fees: Hourly user fee: $20
Hourly rate for technician: $40