Fluorescence microscopy takes advantage of molecules that are capable of fluorescence, which is the absorption of energy from specific wavelengths of light and subsequently emitting (or releasing) light of a longer wavelength. The difference between the peak excitation wavelength and emission wavelength during fluorescence decay is known as “Stokes” shift. A fluorescence microscope utilizes this characteristic shift to generate microscopic images to study unique structures and behaviors in cells and/or tissues. Combined with sensitive cameras, fluorescence microscopy can provide the user with both qualitative and quantitative data. Epifluorescence (wide field) microscopy is ideal for imaging thin samples such as cell monolayers.
School of Pharmacy Confocal Microscope Published Imagery:
Location: Amarillo Research Building
1406 Coulter - 2203B
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Fees: Hourly user fee: $20
Hourly rate for technician: $40