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Cell Physiology and Molecular Biophysics


Raul Martínez-Zaguilán

Curriculum Vitae

Contact Information
Education
Academic Appointments
Awards
Honors
Publications
Abstracts
Societies and Memberships
Committees
Reviewer
Mentoring
UMC Team

Lab Photo: Raul Martínez-Zaguilán; Souad Sennoune; Hesham Attaya; Debasish Maiti; Seema Rawal; Karina Bakunts.


CONTACT INFORMATION
Raul Martínez-Zaguilán
Department of Physiology
Texas Tech University Health Sciences Center
Lubbock, Texas 79430-6551

Phone: (806) 743-2562
FAX: (806) 743-1512
E-mail: Raul.MartinezZaguilan.ttuhsc.edu

EDUCATION

  • 1978-1982, B.Sci. - Univ. Aut. Metrop., Mexico City - Biology - A. Reyes
  • 1983-1986, M.Sci - Univ. Aut. Metrop., Mexico City - Biology - A. Reyes
  • 1987-1988, Colorado State University, Ft. Collins, CO - Biochemistry - R.J. Gillies
  • 1988-1991, Ph.D. - University of Arizona, Tucson, AZ - Biochemistry - R.J. Gillies

ACADEMIC APPOINTMENTS:

  • 1982-1986, Research Assistant, Biochemistry, UIB, Mexico City
  • 1986-1988, Research Assistant, Biochemistry, Colo.St.Univ., Ft. Collins, CO
  • 1988-1991, Research Assistant, Biochemistry, Univ. Arizona, Tucson, AZ
  • 1991-1993, Research Associate, Biochemistry, Univ. Arizona, Tucson, AZ
  • 1993-1994, Assistant Res. Scientist, Biochemistry, Univ. Arizona, Tucson, AZ
  • 1994-1995, Research Associate, Physiology, Univ. Arizona, Tucson, AZ
  • 1996-present, Active Member, Southwest Cancer Center, University Medical Center, Lubbock, TX
  • 1995-2001, Assistant Professor, Dept. Physiology, Texas Tech Univ HSC, Lubbock, TX
  • 2001-2005, Associate Professor, Dept. Physiology, Texas Tech Univ HSC, Lubbock, TX
  • 2001-present, Associate Professor, Southwest Cancer Center, Texas Tech Univ HSC, Lubbock, TX
  • 2005-present, Associate Professor, Dept. of Cell Physiology and Molecular Biophysics,Texas Tech Univ HSC, Lubbock, TX
  • 2011-present, Director, Image Analysis Core Facility, Texas Tech Univ HSC, Lubbock, TX
  • 2013-present, Associate Professor, Adjunct, Dept. Mechanical Engineering, Texas Tech University, Lubbock, TX

Administrative duties in education:

  • Coordinator Physiology Seminar Series 2005-present>
  • Section Director (General Physiology Section) in Medical Physiology (5803), 2000-present
  • Course Director (Topics in Physiology, 6105), 1999-present
  • Scientific Director, Center of Excellence in Imaging of Cell Physiology and Pathophysiology; 2000-present

AWARDS: NATIONAL:

  • National Research Service Award (NIH-HLBI) 1994-1995
  • American Heart Association-National 9750558N; Type: Grant in Aid; “Vacuolar-type Proton ATPases in Human Endothelial Cells” 1/01/98- 12/30/2000 (P.I.)
  • Advanced Technology Program; Texas Higher Education Coordinating Board Type: Research Grant; “Development of fluorescent Probes to Monitor Millimolar Calcium Concentrations in Intracellular Stores” 1/1/98 - 8/30/2000 (P.I.)
  • Underwood Foundation "Proton ATPases in Human Cancer Cells: Its role in Multidrug Resistance and Metastasis" Everado Cobos (P.I.), Multi year grant; subcontract: Raul Martínez-Zaguilán, April 1, 1998-Dec 31, 1998.
  • American Heart Association (National 9950645N): "Mechanisms of ischemia-induced arrhytmias in pacemaker cells isolated from the rabbit sinoatrial node”: Jan 1, 1999 - Jan 2002 (collaborator; Richard D. Nathan - P.I.).
  • Texas Higher Education Coordinating Board/Advanced Research Project (010674-034) “Fluorescent Probes to monitor millimolar calcium contents in intracellular stores." Supplement grant to support a High School Teacher (Amadu Ba, Ph.D. Crosbyton CISD, Crosbyton, TX; Biology teacher): July 1, 1999-August 26, 1999 (P.I.)
  • American Cancer Society; Type: Research Project Grant (RPG-00-035-01-CNE): “Vacuolar type Proton ATPases in Human Breast Cancer Cells” 1/01/2000- 12/31/2002 (P.I.)
  • Texas Higher Education Coordinating Board/Advanced Research Project (010674-034) “Flourescent Probes to monitor millimolar calcium contents in intracellular stores." Supplement grant to support a High School Teacher (Ross Lawson, B.S., Lubbock High, Lubbock TX (Chemistry teacher): June l, 2000-August 11, 2000 (P.I.)
  • National Institutes of Health (NHLBI #1RO1HL/DK656905-01) Type: Research Project Grant; “Proton ATPases in Microvascular Endothelial Cells and Diabetes”; Period 9/01/2000- 8/30/2005 (P.I.)
  • Texas Higher Education Coordinating Board/Advanced Research Project (010674-0012-2001). Research Grant: “Proton Pumps at the Cell Surface are Targets to Halt Angiogenesis and Metastasis in Breast Cancer Cells” 1/1/2002-12/31/2003 (PI)
  • American Heart Association -Texas Affiliate 0555070Y; Grant in Aid; "Endosomes/lysosoems are Ca2+ stores in angiogenesis and daibetes" 7/1-2005-6/30/2007 (P.I.)
  • American Heart Association –National 0630207N Scientist Development Grant; "Vacuolar type H+-ATPases in Angiogenesis" (Collaborator; Souad Sennoune, P.I.) 1/1/2006-12/31/2009
  • American Heart Associate Texas Affiliate (# 0855168F). Greant in Aid. "Proton ATPases in Angiogenesis and Cancer". Martinez-Zaguilan (P.I.) July 1, 2008-June 30, 2010.
  • Cancer Prevention Research Institute of Texas (CPRIT) High Risk/High Impact Grant. "Integrated microfluidic technology for high throughput screening of the metastatic potential of tumor cells". Martinez-Zaguilan, Collaborator, (P.I. Siva Vanapalli). September 1, 2010-August 30, 2012.

INTRAMURAL AWARDS:

  • Institutional Seed Grant "Vacuolar-type Proton ATPases in Microvascular Endothelial Cells" Raul Martínez-Zaguilán (P.I.), March 1, 1997- Feb 28, 1998
  • Institutional Seed Grant “Proton ATPases in Multi Drug Resistant Breast Cancer Cells” Raul Martínez-Zaguilán (P.I.), Everardo Cobos (Collaborator, Southwest Cancer Center) September 1, 1998 - August 30, 1999.
  • Center of Excellence at TTUHSC “Imaging of Cell Physiology and Pathophysiology“ Raul Martínez-Zaguilán (Scientific Director) and John M. Orem (Administrative Director). August 1, 2000 - July 30, 2002
  • School of Pharmacy, TTUHSC Cancer Research Center, Raul Martínez-Zaguilán (collaborator), Suzanne Lindsey (P.I.) Department of Pharmaceutical Sciences, TTUHSC, Pamala Hallin (collaborator), Department of Pharmaceutical Sciences, TTUHSC; and Sheppard (collaborator). August 1, 2000 - July 30, 2001.
  • American Association of Colleges and Pharmacy, Grant in Aid for New Faculty: “Apoptosis induced by an anti-epidermal growth factor receptor antibody in pancreatic cancer” Pamala Hallin (P.I.) and Raul Martinez-Zaguilan (collaborator). Dec 1, 2000 - Nov 30, 2001.
  • Neely Treadwell Award for Cancer Research; UMC/Southwest Cancer Center. “Vacuolar type proton ATPases in human breast cancer cells” January 1, 2005 - December 31, 2005 (PI)
  • Institutional Seed Grant - School of Medicine: "Proton ATPases modulate tumor angiogenesis and tumor size in diabetes". September 2005-June 2007. R. Martinez-Zaguilan (collaborator), Souad Sennoune (P.I.).
  • Institutional Seed Grant - School of Medicine: "Hypoxia induces vascular H+-ATPase in human coronary microvascular endothelial cells and promotes angiogenesis". July 1, 2010-June 30, 2011. R. Martinez-Zaguilan, P.I.

Honors:

  • Neely Treadwell Cancer Investigator Award, 2004
  • Chancellors Award for Excellence in Research, Lubbock, TX, 2001.
  • American Cancer Society, Local chapter, Lubbock, TX, 2000
  • Travel Award, Young Vascular Investigator, San Antonio, TX, 1999
  • Travel Award, Young Vascular Investigator, San Antonio, TX, 1997.
  • Travel Award,TEXAN Conference, Austin, TX, 1997.
  • National Research Service Award. NIH-HLBI, 1994-1995
  • Travel Award, ATPase Conference, New York National Academy of Sciences Conference, 1992
  • Travel Award, Aspen Cancer Conference, Aspen CO, 1991
  • Best Dissertation Award:      "The Role of Intracellular pH and Calcium in the Regulation of Cellular Functions". Department of Biochemistry, Univ. of Arizona, 1991 (R.J. Gillies, advisor).

PUBLICATIONS:

  • Reyes A, Chavarria ME, Martinez R. (1983). (Biochemistry of the male genital tract). Gac. Med. Mex. 119: 139-145.
  • Reyes A, Martinez R., Luna M, Chavarria ME, Merino G (1984). Quantitative evaluation of the human spermatozoal motility and acrosome reaction in infertile oligozoospermic men and fertile euspermic men. Arch. Androl. 12: 187-194.
  • Villegas H, Martinez R., Chavarria ME, Reyes A (1987). (Ultrastructural analysis of spermatozoa in euspermic fertile and oligozoospermic infertile men). Ginec. Obstet. Mex. 55: 41-46.
  • Reyes A, Martinez R., Luna M, Chavarria ME (1987). Concentrations of calmodulin in sperm in relation to their motility in fertile euspermic and infertile asthenozoospermic men. Int. J. Androl. 10: 507-515.
  • Martinez R., Gillies RJ, Giuliano KA (1988). Effect of serum on the intracellular pH of BALB/c-3T3 cells: Serum deprivation causes changes in the sensitivity of cells to serum. J. Cell. Physiol. 136: 154-160.
  • Lucas CA, Gillies, RJ, Olson JE, Giuliano KA, Martinez R., Sneider JM (1988). Intracellular acidification inhibits the proliferative response in BALB/c-3T3 cells. J. Cell. Physiol. 136: 161-167.
  • Gillies RJ, Martinez R., Sneider JM, Hoyer PB (1989). Sphingosine inhibits phorbol 12-myristate 13-acetate-, but not serum-induced activation of Na+/H+ exchange in mammalian cells. J. Cell. Physiol. 139: 125-130.
  • Martinez R., Gillies RJ (1989). Dimethylsulfoxide modifies the sensitivity of BALB/c-3T3 cells to the activation of Na+/H+ exchange by phorbol esters. J. Cell. Physiol. 139: 131-135.
  • Gillies RJ, Martínez-Zaguilán R, Martinez GM, Perona R, Serrano R (1990). Tumorigenic 3T3 cells maintain an alkaline intracellular pH under physiological conditions. Proc. Natl. Acad. Sci. 87: 7414-7418.
  • Reyes A, Martínez-Zaguilán R, Luna M, Chavarria ME (1991). (Biochemistry of the testicular function). In: Selected Topics in Reproductive Biology. Dominguez, R. (ed.), Miguel Angel Porrua Press, p. 195-230.
  • Wegner JA, Martínez-Zaguilán R, Wise ME, Gillies RJ, Hoyer PB (1990). Prostaglandin F2a-induced calcium transients in ovine large luteal cells: I. Alterations of cytosolic-free calcium levels and calcium flux. Endocrinology 127: 3029-3037.
  • Martínez-Zaguilán R, Martinez GM, Lattanzio F, Gillies RJ (1991). Simultaneous measurement of intracellular pH and Ca2+ using the fluorescence of Snarf-1 and Fura-2 in BALB/c-3T3 cells. Am. J. Physiol. (Cell Physiol.) 260:C297-C307.
  • Gillies RJ, Martínez-Zaguilán R (1991). Regulation of intracellular pH in BALB/c-3T3 cells: Bicarbonate raises pH via NaHCO3/HCl exchange and attenuates the activation of Na+/H+ exchange by serum. J. Biol. Chem. 266:1551-1556.
  • Wegner JA, Martínez-Zaguilán R, Gillies RJ, Hoyer PB (1991). Prostaglandin F2alpha-induced calcium transients in ovine large luteal cells: II. Modulation of the transient and resting cytosolic free calcium alters progesterone secretion. Endocrinology, 128: 929-936.
  • García R, Martínez R,. Rábago M, Hernández-Pérez O, Reyes A, Rosado A (1991). Subcellular distribution of phospholipase A2 and ATPases during capacitation and acrosome reaction in guinea pig spermatozoa. Arch. Androl. 26: 93-105.
  • Gillies RJ, Scherer PG, Raghunand N, Okerlund LS, Martínez-Zaguilán R, Hesterberg L, Dale B (1991). Iteration of hybridoma growth and productivity in hollow fiber bioreactors using 31P NMR. Mag. Reson. Med. 18:181-192.
  • Martínez-Zaguilán R, Gillies RJ (1992). A plasma membrane V-type H+-ATPase may contribute to elevated intracellular pH (pHin ) in some human tumor cells. Ann. N.Y.Acad.Sci., 671: 478-480.
  • Martínez-Zaguilán R (1992). Measurement of intracellular Ca2+ and pHin in cultured cells by fluorescence spectroscopy. In: Vitro Methods in Toxicology and Pharmacology. Watson R (ed.). CRC Press, p 217-236.
  • Gillies RJ, Martínez-Zaguilán R, Peterson EP, Perona R (1992). The role of intracellular pH in mammalian cell proliferation. Cell. Physiol. Biochem. 2: 159-179.
  • Martínez-Zaguilán R, Martínez GM, Lynch RM, Gillies RJ (1993). Vacuolar-type H+ ATPase are functionally expressed in plasma membranes of human tumor cells. Am. J. Physiol. (Cell Physiol.) 265: C1015-C1029.
  • Wegner JA, Martínez-Zaguilán R, Gillies RJ, Hoyer PB (1994). Prostaglandin F2alpha releases intracellular calcium from a thapsigargin-sensitive pool in ovine large luteal cells. Am. J. Physiol. (Endocrinol. Metab.) 266:E50-E56.
  • Gunn M, Martínez-Zaguilán R, Walden-Hopkins S, Woolridge D, Gillies RJ (1994). NIH-3T3 cells transfected with a yeast H+-ATPase have altered rates of protein turnover. Arch. Biochem. Biophys. 314:268-275.
  • Sanchez-Armass S, Martínez-Zaguilán R, Martinez GM, Gillies RJ (1994). Regulation of pH in rat brain synaptosomes: Role of bicarbonate and potassium. J. Neurophysiol. 71: 2236-2248.
  • Martínez-Zaguilán R, Gillies RJ, Sanchez-Armass S (1994). Role of Cl- in the regulation of cytosolic pH in rat brain synaptosomes. J. Neurophysiol. 71: 2249-2257.
  • Peterson E, Martínez-Zaguilán R, Martinez GM, Perona R, Gillies RJ (1994). NIH 3T3 cells transfected with a yeast H+-ATPase have altered sensitivity to insulin, IGF-I and PDGF AA. J. Cell Physiol. 159: 551-560.
  • Wysynski A-M, Martínez-Zaguilán R, Alden C, Gandolfi AJ, Gillies RJ (1994). Phenobarbital induces cytosolic acidification in an established liver epithelial cell line. Toxicol. Lett. 74:157-166.
  • Lax D, Martínez-Zaguilán R, Gillies RJ. (1994). Furazolidone increases thapsigargin sensitive Ca2+-ATPase in chick cardiac myocytes. Am. J. Phsyiol. (Heart Circ.Physiol) 267: H734-H741.
  • Martinez GM, Martínez-Zaguilán R, Gillies RJ (1994). Effect of glucose on pHin and [Ca2+]in in NIH-3T3 cells transfected with the yeast P-type-H+-ATPase. J. Cell Physiol. 161:129-141.
  • Martínez-Zaguilán R, Wegner J, Gillies RJ, Hoyer P (1994). Differential regulation of Ca2+ homeostasis in ovine large and small luteal cells. Endocrinology 135: 2099-2108.
  • Martínez-Zaguilán R, Tompkins LS, and Lynch R.M. (1994). Simultaneous analysis of multiple fluorescent probes in single cells by microspectroscopic imaging. Proc. Int. Soc. Optical Engin., S.P.I.E. 2137:17-28.
  • Lien Y-HH, Wang X, Gillies RJ, Martínez-Zaguilán R (1995). Modulation of intracellular free calcium by glucose in madin-darby canine kidney cells: Role of endoplasmic reticulum Ca2+-ATPase. Am. J. Physiol. (Renal Fluid and Electrolyte Physiol) 268: F671-F679.
  • Wang X, Lien Y-HH, Gillies RJ, Martínez-Zaguilán R (1995). Glucose decreases intracellular calcium ([Ca2+]in) and pH (pHin) in cultured rat vascular smooth muscle cells: Role of Ca2+-ATPase. Cell. Physiol. Biochem. 5:299-312.
  • Lien Y-HH, Wang X, Martínez-Zaguilán R (1995). Differential effects of endoplasmic reticulum Ca2+-ATPase inhibitors on Madin Darby Canine Kidney (MDCK) cells. Cell. Physiol. Biochem. 5:371-379.
  • Martínez-Zaguilán R, Seftor EA, Chu Y-W, Hendrix MJC, Gillies RJ (1996). Acidic pH enhances the invasive behavior of human melanoma cells. Clin. Exp. Metastasis. 14:176-186.
  • Martínez-Zaguilán R, Gurule, WM, Lynch RM (1996). Simultaneous measurement of calcium and pH in insulin secreting cell lines by fluorescence spectral imaging. Am. J. Physiol. 270 (Cell Physiol., 39) C1438-C1446.
  • Martínez-Zaguilán R, Parnami, G, Lynch RM. (1996). Selection of fluorescent ion indicators for simultaneous measurements of pH and Ca2+. Cell Calcium. 19:337-349.
  • Martínez-Zaguilán R, Chinnock BF, Wald-Hopkins S, Bernas M, Way D, Witte MH, Gillies RJ (1996). [Ca2+]in and pHin homeostasis in kaposi sarcoma cells. Cell. Physiol.Biochem. 6:169-148.
  • Martínez-Zaguilán R, Wesson DE. (1996). Regulation of endoplasmic reticulum(ER)-Ca2+-ATPase by glycolysis in eukaryotic cells. Min. Electrolyte Metab. 22:318-335.
  • Martínez-Zaguilán R, Tompkins LS, Gillies RJ, Lynch RM (1999). Simultaneous analysis of intracellular pH and Ca2+ from cell populations. Methods Molec.Biol. 114:287-306.
  • Martínez-Zaguilán R, Parnami J, Martinez GM (1998). Mag-Fura-2 (Furaptra) exhibits both low (uM) and high (nM) affinity for Ca2+. Cell. Physiol. Biochem. 8: 158-174.
  • Sarvazyan N, Swift L, Martínez-Zaguilán R (1998). Effect of oxidants on properties of fluorescent calcium indicators. Arch. Biochem. Biophys. 350: 132-136.
  • Martínez-Zaguilán R, Martinez GM, Gomez A, Hendrix MJC, Gillies RJ (1998). Distinct rgulation of pHin and [Ca2+]in in human melanoma cells with different metastatic potential. J. Cell. Physiol. 176: 196-205.
  • Hudson CA, Rojas JS, Sarvazyan N, Wesson DE, Martínez-Zaguilán R (1998). Interactions between benzylamiloride and Fura-2: Studies in vitro and in cardiac myocytes. Arch. Biochem. Biophys. 356: 25-34.
  • Simoni J, Simoni G, Martínez-Zaguilán, R, Wesson d, Lox CD, Prien SD, Kumar V. (1998) Improved blood substitute: Evaluation of its effects on human endothelial cells. ASAIO J 44:M356-M367
  • Rojas JG, Sanka S, Györke S, Wesson DE, Minta A, Martínez-Zaguilán R (1999). Intracellular pH (pHin) and Cytosolic Calcium ([Ca2+]cyt) Regulation via ATPases: Studies in Cell Populations, Single Cells and Subcellular Compartments. Proc.Int.Soc.Optical Engin., S.P.I.E. 3600:000-000
  • Martínez-Zaguilán R. (1999) Angiostantin's partners. Science, 284: 433-434
  • Martínez-Zaguilán R, Raghunand N, Lynch RM, Bellamy W, Martinez GM, Rojas B, Smith D, Dalton WS, Gillies RJ (1999). pH and drug resistance I: Plasmalemmal V-type H+ ATPase is functionally expressed in drug resistant human breast carcinoma cell lines. Biochem. Pharmacol. 57:1037-1046.
  • Raghunand N, Martínez-Zaguilán R, Wright SH, Gillies RJ (1999). pH and drug resistance II: Turnover of acidic vesicles and resistance to weakly basic chemotherapeutic drugs. Biochem. Pharmacol. 57:1037-1046.
  • Meininger CJ, Marinos RS, Hatakeyama K, Martínez-Zaguilán R, Rojas JD, Kelly KA and Wu G (2000). Impaired nitric oxide production in coronary endothelial cells of the spontaneously diabetic BB rat is due to tetradihydrobiopterine deficiency. Biochem J. 349:353-356.
  • Rojas JG, Sanka SC, Luo D, Martinez GM, and Martínez-Zaguilán R (2000). Proton pumps, angiogenesis and metastatic breast cancer. Proc. Int. Soc. Optical Engin., S.P.I.E., 3924:67-78.
  • Sanka SC, Bennett D, Rojas JR, Tasby G, Meininger CJ, Wu G, Wesson DE, and Martínez-Zaguilán R (2000). Ca2+ homeostasis in microvascular endothelial cells from an insulin-dependent diabetic model: Role of endosomes/lysosomes. Proc. Int. Soc. Optical Engin., S.P.I.E., 3924:56-66.
  • Martinez GM, Gollahon LS, Shafer K, Oomman SK, Busch C, Martinez-Zaguilan R. (2001). Fluorescent pH probes, fluorescent proteins, and intrinsic cellular fluorochromes are tools to study cytosolic pH (pHcyt) in mammalian cells. Proc. Int. Soc. Opt. Engin., SPIE., 4259 :144-156
  • Sanchez-Armass S., Sennoune S., Martinez G.M., Ortega F., Martinez-Zaguilan R. (2002) Cytosolic pH gradients in cultured neuronal cell lines studied by laser scanning confocal microscopy, real time confocal microscopy, and spectral imaging microscopy. Proc. Int. Soc. Opt. Engin., SPIE., 4626:513-522.
  • Sennoune S., Maiti D., Martinez-Zaguilan R. (2003) Analysis of intracellular pH (pHcyt) in mouse models of angiogenesis and carcinogenesis by spectral imaging microscopy, real time confocal imaging and multiphoton spectral imaging. Proc. Int. Soc. Opt. Engin., SPIE., 4967 :135-145
  • Sennoune S., Luo D., Martinez-Zaguilan R. (2004) Plasmalemmal vacuolar-type H+-ATPase in cancer biology. Cell Biochemistry and Biophysics, 40(2) :185-206.
  • Sennoune S, Bakunts K, Martinez GM, Chua-Tuan JL, Kebir Y, Attaya MN, Martinez-Zaguilan R. (2004) Vacuolar H+-ATPase in human breast cancer cells with distinct metastatic potential, distribution and functional activity. Am. J. Physiol. – Cell Physiol., 286(6) :C1443-1452.
  • Rojas JD, Sennoune S, Martinez GM, Bakunts K, Meinenger CJ, Wu G, Wesson DE, Seftor EA, Hendrix MJC, Martinez-Zaguilan R. (2004) Plasmalemmal vacuolar-type H+-ATPase is decreased in microvascular endothelial cells from a diabetic model. J. Cell Physiol., 201: 190-200.
  • Rojas JD, Sennoune SR, Maiti D, Martinez GM, Bakunts K, Wesson DE, Martinez-Zaguilan R. (2004) Plasmalemmal V-H+-ATPases regulate intracellular pH in human lung microvascular endothelial cells. Biochem. Biophys. Res. Commun., 320: 1123-1132.
  • Martinez-Zaguilan R, Tompkins LS, Gillies RJ, Lynch RM. Simultaneous analysis of intracellular pH and Ca2+ from cell populations. Meth. Mol. Biol, 312: 269-288, 2005.
  • Sanchez-Armass S*, Sennoune SR*, Ortega F, Martinez-Zaguilan R. Spectral imaging microscopy demonstrates cytoplasmic pH oscillations in glial cells. Am. J. Physiol – Cell Physiol, 290(2):C524-538, 2006 (*These authors contributed equally to this work)
  • Shiner EK, Terentyev D, Bryan A, Sennoune S, Martinez-Zaguilan R, Li G, GyörkE S, Williams SW, Rumbaugh KP. Pseudomonas aeruginosa autoinducer modulates host cell responses through calcium signaling. Cellular Microbiology, 2006, in press.
  • Rojas JD*, Sennoune SR*, Maiti D, Bakunts K, Reuveni M, Sanka SC, Martinez GM, Seftor EA, Meininger CJ, Wu G, Wesson DE, Hendrix MJ, Martinez-Zaguilan R. Vacuolar Type H+-ATPases at the plasma membrane regulate pH and cell migration in microvascular endothelial cells. Am. J. Physiol –Heart Circ Physiol, 291: H1147-H1157, 2006 (*These authors contributed equally to this work).
  • Sennoune, SR, Martinez-Zaguilan, R. Plasmalemmal Vacuolar H+-ATPases in Angiogenesis and Cancer. J Bioenerg Biomembr, 39: 427-433, 2007.
  • Hinton, A., Sennoune, S.R., Bond, S., Fang, M., Reuveni, M., Sahagian, G.G., Jay, D., Martinez-Zaguilan, R., Forgac, M. Function of a subunit isoforms of the V-ATPases in pH homeostasis and in vitro invasion of MDA-MB231 human breast cancer cells. J. Biol. Chem., 284: 16400-16408, 2009. 
  • Sennouns, S.R., Martinez-Zaguilan, R. Vacuolar H+-ATPase signaling pathway in cancer. Curr. Protein Pept. Sci., 13:152-163, 2012. 
  • Bastatas, L., Martinez-Marin, D., Mathews, J., Hashem, J., Lee, Y.J., Sennoune, S., Filleur, S., Martinez-Zaguilan, R., Park, S. AFM nano-mechanics and calcium dynamics of prostate cancer cells with distinct metastatic potential. Biochim Biophys Acta 1820:1111-1120, 2012. 
  • Martinez-Zaguilan, R., Tompkins, L.S., Gillies, R.J., Lynch, R.M. Simultaneous analysis of intracellular pH and Ca2+ from cell populations. Methods Mol Biol 937:253-271, 2013. 
  • Helmer, R.A., Martinez-Zaguilan, R., Dertine, J.S., Fulford, C., Foreman, O., Peiris, V., Chilton, S. Helicase-like transcription factor (Hltf) regulates G2/M transition, Wt1/Gata4/Hif-1a cardiac transcription networks, and collagen biogenesis. PLOS One 8:e-80461-80461, 2013. 
  • Sennoune, R.S., Arutunyan, A., Del Rosario, C., Castro-Marin, R., Hussain, F., Martinez-Zaguilan, R. Simultaneous V-ATPase regulates communication between microvascular endothelial and metastatic cells. Cell Molec Biol, 2014, in press. 

Invited Reviews:

  • Reyes A, Martínez-Zaguilán R, Luna M, Chavarria ME (1991). (Biochemistry of the testicular function). In: Selected Topics in Reproductive Biology. Dominguez, R. (ed.), Miguel Angel Porrua Press, p. 195-230.
  • Martínez-Zaguilán R (1992). Measurement of intracellular Ca2+ and pHin in cultured cells by fluorescence spectroscopy. In: Vitro Methods in Toxicology and Pharmacology. Watson R (ed.). CRC Press, p 217-236.
  • Martínez-Zaguilán R, Tompkins L, Gillies RJ, Lynch RM. Simultaneous measurements of intracellular pH and Ca2+ from cell populations. Meth. Mol. Biol, 1999, 114: 287-306.
  • Martínez-Zaguilán R, Tompkins L, Gillies RJ, Lynch RM. Simultaneous measurements of intracellular pH and Ca2+ from cell populations. Meth. Mol. Biol, 2005, 312: 269-287.

ABSTRACTS:

  • Hoyer PB, Wegner JA, Martínez-Zaguilán R, Gillies RJ. Critical Levels of cytosolic-free calcium relate to optimal secretion of progesterone in ovine large luteal cells. Endocrine Soc. 72nd Ann. Meet. 965A, 1990.
  • Hoyer PB, Wegner JA, Martínez-Zaguilán R, Gillies RJ. Differential regulation of cytosolic free calcium ([Ca2+]I) in ovine small (SLC) and large (LLC) luteal cells. Proceedings of VII Ovarian workshop, Maryville, TN (Abstract #20), 1990.
  • Martínez-Zaguilán R, Martínez GM and Gillies RJ (1990) Differential effects of H+-ATPase inhibitors on intracellular pH. Department of Physiology, Univ of Arizona. May 23, 1990, Tucson AZ.
  • Sánchez-Armass S, Martínez-Zaguilán R, Gillies RJ. (Role of HCO3-/Cl- exchange in the regulation of intracellular pH (pHi) in synaptosomes. XVIII Congreso Nacional de la Sociedad Mexicana de Bioquímica. San Luis Potosí, Mexico. November 11-16, 1990.
  • Martínez-Zaguilán R, Martínez GM, Gillies RJ (1991) Possible involvement of a vacuolar type H+-ATPase in the maintenance of a high intracellular pH in human tumor cells. XXXV Annual Meeting of the American Biophysical Society. February 24-28, 1991. Biophys J 59: 562a
  • Sánchez-Armass S, Martínez-Zaguilán R, Gillies RJ. Role of HCO3- in the regulation of intracellular pH in rat brain synaptosomes. XXXV Annual Meeting of the American Biophysical Society. February 24-28, 1991. Biophys J 59: 590a
  • Gillies RJ, Martínez-Zaguilán R, Perona R (1991). Molecular controls of intracellular pH. The Sixth Aspen Cancer Conference: Molecular Mechanisms of Genetic Deregulation in Toxicity and Carcinogenesis. Aspen Colorado, July 20-25, 1991.
  • Martínez-Zaguilán R, and Gillies RJ (1991). Evidence for the presence of a vacuolar type H+-ATPase in the plasma membrane of some human tumor cells. Sixth Aspen Cancer Conference. Aspen Colorado, July 20-25, 1991.
  • Sánchez-Armass S, Martínez-Zaguilán R, Gillies RJ. XXXIV Congreso Nacional de Ciencias Fisiológicas. September 1991, Colima México, MEXICO
  • Sánchez-Armass S, Martínez-Zaguilán R, Gillies RJ. (Regulation of the activity of the Na+/H+ exchanger by Ca2+ in synaptosomes). Congreso Mexicano de Bioenergética. November 1991, Taxco Morelos, México.
  • Martínez-Zaguilán R, and Gillies, R.J. A plasma membrane V-type H+-ATPase contributes to elevated intracellular pH in some human tumor cells. The New York Academy of Sciences. Ion-Motive ATPases: Structure, Function and Regulation. June 13-17, 1992. Cleveland, Ohio. Ann.N.Y.Acad.Sci., 671: 478-480.
  • Gillies and , Martínez-Zaguilán R. Evidence for expression of V-type H+-ATPase in plasma membranes of cultured human cells. The American Society for Cell Biology. Thirty-Second Annual Meeting. Nov.,15-19, 1992. Denver, Colorado. Mol. Biol. Cell., 3:123a
  • Chinnock, B., Martínez-Zaguilán R,, Wald-Hopkins, S., Witte, M., Way, D., Gillies, R.J. Altered calcium homeostasis in Kaposi's sarcoma cells. The American Society for Cell Biology. Thirty-third Annual Meeting. June 1993, Sand Diego, California.
  • Sánchez-Armass S, Martínez-Zaguilán R, Gillies RJ. The cytosolic pH in synaptosomes is increased by Cl- removal or high K+ solution. The Biophysical Society. 37th Annual Meeting. 14-18 February, 1993. Washington, D.C., Biophys.J., 64:A401
  • Martínez-Zaguilán, R, Chinnock, B., Wald-Hopkins, S., Way, ., Witte, M, Gillies, R.J. Calcium and pH homeostasis in Kaposi sarcoma cells. Thirty-Ninth Annual Meeting Western Society for Clinical Investigation., 9-12 February, 1994. Carmel, California., Clin.Res., 42:31A
  • Lien, Y.-H.H. Wang, X., Gillies, R.J., and Martínez-Zaguilán R. Modulation of intracellular free calcium by glucose in madin-darby canine kidney cells: Effects of endoplasmic reticulum Ca2+-ATPase and capacitative calcium entry. Thirty-Ninth Annual Meeting Western Society for Clinical Investigation., 9-12 February, 1994. Carmel, California., Clin.Res., 42:94A
  • Martínez-Zaguilán R, Linda, S., and Lynch, R.M. Simultaneous analysis of multiple fluorescent probes in single cells by microspectroscopic imaging. The Society of Photo-Optical Instrumentation Engineers (SPIE)- International Society for Optical Engineering. 24-26 January, 1994., Los Angeles, California., Int.Soc.Optical Engin., (SPIE) 2137:17-28.
  • Martínez-Zaguilán R, and Lynch, R.M., Simultaneous measurement of calcium and pH in single insulin secreting cells by fluorescence spectroscopy. The Biophysical Society. 38th Annual Meeting. 6-10 March, 1994. New Orleans, Louisiana, D.C., Biophys.J., 66:A162
  • Lynch, R.M., Laughrey, L., and Martínez-Zaguilán R. Measurement of intravesicular (IVES) Ca2+ and pH in single smooth muscle cells using spectral imaging. The Biophysical Society. 39th Annual Meeting. 12-16 February, 1995. San Francisco, California, Biophys.J., 68:A113.
  • Martínez-Zaguilán R,Parnami, G., and Lynch, R.M. Fluorescence energy transfer (FET) between Fluo-3 and SNARF-1 affects the accuracy of ion measurements when using these probes simultaneously. The Biophysical Society. 39th Annual Meeting. 12-16 February, 1995. San Francisco, California, Biophys.J., 68:A195.
  • Wang, X., Lien, Y.-H.H., Gillies, R.J., and Martínez-Zaguilán R. Glucose decreases intracellular calcium and pHin in cultured vascular smooth muscle (VSM) cells. The Biophysical Society. 39th Annual Meeting. 12-16 February, 1995. San Francisco, California, Biophys.J., 68:A281.
  • Rojas, JD. Hudson, CA. Sarvazyan, N. Wesson, DE. Martínez-Zaguilán, R. (1997) Interactions between amiloride derivatives (ami-d) and fura-2: studies in vitro and in cardiac myocytes. Biophys J, 72:A202.
  • Rojas, JD. Martínez, GM. Bezner,A. Meininger, CJ. Wu, G. Wesson, DE. Martínez-Zaguilán, R. (1997) Plasmalemmal vacuolar type H+ ATPases regulate intracellular pH in microvascular coronary endothelial cell. Biophys J, 72:A289.
  • Rojas, JD. Martínez, GM. Bezner,A. Meininger, CJ. Wu, G. Wesson, DE. Martínez-Zaguilán, R. Intracellular pH (pHin) regulation in microvascular coronary endothelial cells (mcec): role of plasmalemmal vacuolar type H+ ATPases (pm v-ATPASES). Texas Society of Academic Nephrologists (TXSAN) 4th Annual Meeting, May 11, 1997.
  • Rojas, JD. Wesson, DE. Meininger, CJ. Wu, G. and Martínez-Zaguilán, R. (1998). Plasmalemmal vacuolar type H+-ATPase is decreased in microvascular coronary endothelial cells from an insulin dependent diabetic model. FASEB J. 12:A1024.
  • Martínez-Zaguilán, R. Rojas, JD. Sanka, S. (1999). Intracellular pH (pHin) and cytosolic ([Ca2+]cyt) regulation via ATPases: studies in cell populations, single cells, and subcellular compartments. Society of Photo-Optical Instrumentation Engineers Annual Meeting. January 23-29,1999. San Jose, California.
  • Rojas, JD. Martínez, GM. Tasby, GB. Hudson, CA. Wesson, DE. and Martínez-Zaguilán, R. (1999). Identification of a novel intracellular pH regulation in human lung microvascular endothelial cells: plasmalemmal vacuolar type proton pumps. FASEB J. 13:A501.
  • Rojas, JD Meininger, CJ. Wu Martínez-Zaguilán, R. (1999). Plasmalemmal vacuolar type H+-ATPASES (pm-V-H+-ATPases) are involved in angiogenesis in microvascular endothelial cells. FASEB J. 13:A528.
  • Martínez-Zaguilán, R. Seftor, E. Hendrix, MJC. Meininger, CJ. Wu, G. Wesson, DE. Rojas, JD. (1999). Intracellular pH (pHin) Regulation and invasion in microvascular coronary endothelial cells. FASEB J. 13:A9.
  • Cheng H, Mallerino MC, Martinez GM, Zaunbrecher G, Cobos E, and Martínez-Zaguilán, R. (1999). Plasmalemmal vacuolar type proton ATPases are involved in multidrug resistance (MDR). Proc Am Asoc Cancer Res 40: 671
  • Rojas, J.D., Sanka, SC, Hendrix MJC, Meininger CJ, Wu G, Seftor EA, Martinez GM and Martínez-Zaguilán, R. (2000) Vacuolar type proton ATPases in angiogenesis and cancer. Proc Am Asoc Cancer Res 41: 303A
  • Rojas, JD, Sanka SC, Hendrix MJC, Seftor E, Attaya HN, Meininger CJ, Wu G, Wesson DE and Martínez-Zaguilán, R (2000). Plasmalemmal V-H+-ATPase (pmV-ATPase) is essential for migration in microvascular endothelial cells. FASEB J, 14:A145
  • Sanchez-Armass S, Sanka SC, Rojas JD and Martínez-Zaguilán, R (2000). Cytosolic pH (pHcyt) gradients in neuroblastoma and glioma cell lines studied by confocal/spectral imaging microscopy. FASEB J, 14:A357
  • Watts MA, Jackson MY, Martinez GM and Martínez-Zaguilán, R. (2000) Calcium and calmodulin kinase (CaM-K) regulate plasmalemmal vacuolar type H+-ATPase in human breast cancer cells. FASEB J, 14:A332
  • Martinez GM, Kuenhel HE, and Martínez-Zaguilán, R (2000). In vitro and in situ characterization of a novel ratiometric pH fluorescent indicator, BCPCF. FASEB J, 14:A357
  • Attaya HN, Hudson CA, Rojas JD, Meininger CJ, Wu G, and Martínez-Zaguilán, R (2000). Plasmalemmal vacuolar type H+-ATPase (pmV-ATPase) in microvascular endothelial cells is regulated by protein kinase C (PKC) and calmodulin kinase (CaM-K). FASEB J, 14:A357
  • Nash J.E., Sanka S.S., Lawson R, Martinez, GM., Meininger CJ., Wu G., and Martinez-Zaguilan R. (2001). Calcium stores in microvascul;ar endothelial cells from a spontaneous of daiabets are altered. FASEB J 15:A116.
  • Luo, D., Sanka SS., Rojas JD., Martinez G., Watts M., and Martinez-Zaguilan R (2001). Intracellular pH gradient in metastatic breast cancer cells is determined by plasmalemmal vacuolar type proton ATPases. FASEB J 15: A444.
  • Attaya HN, Martinez GM, Gada RP, and Martinez-Zaguilan R. (2001) Human metastatic pancreatic cancer cells express plasmalemmal vacuolar type proton ATPases (pmV-ATPases). FASEB J 15: A447.
  • Attaya, N., Meininger, C., Wu, G., and Martinez-Zaguilan, R (2002). Ca2+ release from endosomes/lysosomes (E/L) contributes to their fusion with the plasma membrane. FASEB J., 16: A1155
  • Attaya MN, Attaya HN, Martinez GM, Meininger CJ, Wu G, Martinez-Zaguilan R (2003). Protein kinase C activates plasmalemmal vacuolar type proton ATPase (pm-V-ATPase) in microvascular endothelial cells. FASEB J, 17:A501.
  • Sennoune S, Maiti D, Bakunts K, Martinez GM and Martinez-Zaguilan R. Proton ATPases, angiogenesis and metastasis. FASEB J. 17: A1347, 2003.
  • Sennoune S, Maiti D, Martinez-Zaguilan R. Analysis of intracellular pH (pHcyt) in mouse models of angiogenesis and carcinogenesis by spectral imaging microscopy, real time confocal imaging and multiphoton spectral imaging. Photonic West, Biomedical Optics, San Jose, CA, January 28, 2003.
  • Maiti D, Sennoune S, and Martinez-Zaguilan R. Proton gradients in human breast cancer cells determined by confocal, multiphoton, and spectral imaging microscopy. FASEB J. 17: A467, 2003.
  • Attaya MN, Maiti D, Sennoune SR, Attaya HN, Bakunts K, Martinez-Zaguilan R. Endosomes/lysosomes fuse with the plasma membrane and allow insertion of V-ATPase into the plasma membrane and increase in surface area needed for migration. FASEB J 18:A1023, 2004.
  • Sennoune S, Chua-Tuan J, Bakunts K, Martinez GM, Seftor S, Hendrix MJC and Martinez-Zaguilan R. Plasmalemmal vacuolar-type H+-ATPase is involved in the decreased of the tumor progression in a diabetic model. FASEB J. 17:A1347, 2003
  • Maiti D, Sennoune SR Martinez-Zaguilan R. Ultraviolet (UV-B) light elicits cytosolic acidosis in human breast cancer cells. FASEB J, 18: A188, 2004.
  • Sennoune SR, Maiti D, Martinez-Zaguilan R. Proton waves and gradients in angiogenesis and cancer. FASEB J, 18:A1023, 2004.
  • Sennoune SR, Maiti D, Martinez-Zaguilan R. Proton waves and gradients in angiogenesis and cancer: Biological significance. Biophysics, Baltimore February 18th 2004.
  • Chua-Tuan JL, Sennoune SR, Bakunts K, Martínez GM, Kebir Y, Martínez-Zaguilán R. Vacuolar H+-ATPase in human breast cancer cells with distinct metastatic potential, distribution and functional activity. FASEB J, 18:A188, 2004.

SOCIETIES AND MEMBERSHIPS:

  • Biophysical Society, 1988
  • American Society for Biochemistry and Molecular Biology, 1989
  • Sociedad Latinoamericana de Biofisica, 1989
  • Sociedad Mexicana de Bioquimica, 1990
  • American Physiological Society, 1996
  • American Heart Association, 1998
  • American Cancer Society, 1999

COMMITTEES:

  • Career Opportunities in Physiology Committee, 1998-2000
  • American Heart Association Western Review Consortium peer review committee, 2000-2004

DEPARTMENT COMMITTEES

  • Confocal microscope committee in the Department of Physiology, 1998-present
  • Multiphoton System Dept Physiology, 2002-present
  • Faculty Search Committee, 1999
  • Graduate Faculty Advisory Committee, 2000-present
  • American Heart Western Consortium, 2000-2004

SCHOOL OF MEDICINE COMMITTEES:

  • School of Medicine Admissions Committee, 1996-present

STATE, NATIONAL AND INTERNATIONAL ORGANIZATIONS:

  • Conference Organizer, Chair (1999-2003): "Reporters, Dyes, Reagents and Instrumentation". This is part of the International Conference organized by the International Society for Optical Engineers, SPIE (attended by 12,000 scientists).

Ad Hoc Consultant:

  • Axiom Biotechnologies, Inc., San Diego CA

REVIEWER

  • American Journal of Kidney Diseases
  • American Journal of Physiology (Cell Physiology)
  • American Journal of Physiology (Heart and Circ Physiol)
  • American Journal of Physiology (Endocrinol Metab)
  • American Journal of Physiology (Renal Fluid Electrolyte Physiol)
  • Biochimica et Biophysica Acta
  • Biophysical Journal
  • Cancer Research
  • Journal of Cellular Physiology
  • Journal of Biological Chemistry
  • Journal of the American Society of Nephrology
  • Journal of Histochemistry and Cytochemistry
  • LifeSciences

MENTORING:

  • Graduate Student Mentoring:
    • Somchit Eiam-Ong, Physiology, TTUHSC; Ph.D. Dissertation Committee, 1995-1998
    • Grattendick Kenneth, Microbiology, TTUHSC, Ph.D. Dissertation Committee, 1996-1999
    • Jose D. Rojas, Physiology, TTUHSC, Ph.D. Dissertation Committee, 1997-2000
    • Shankar Sanka, Physiology, TTUHSC, M.S. Thesis Committee, 1998-2000
    • Michelle Watts, Interdisciplinary Studies, TTU, M.S. Thesis Committee, 1998-1999
    • Defeng Luo, Physiology, TTUHSC, Ph.D. Dissertation Committee, 1999-2001
    • Namit Gaur, Chemical Engineer, TTU, M.S. Thesis Committee, 1999-2000
    • Du Zhiqiang, Biology, TTU, Ph.D. Dissertation Committee, 2001-present
  • Postdoctoral Fellow/Research Associates:
    • Jose D. Rojas, Ph.D., Spring-Summer 2000
    • Souad R. Sennoune, Ph.D., 2001-2003
    • Debasish Maiti, Ph.D., 2002-2004
    • Seema Rawal, Ph.D., Summer 2004
  • TTU Graduate Research Assistants:
    • Michelle Watts (Chemistry), Sept - Dec 1998
  • Undergraduate Student mentoring:
    • Howard Hughes Fellows:
      • Sean Howell, May 1996-Feb 1997
      • Amanda Bezner, May 1996-Dec 1997
      • David Bennett, Feb 1998-Dec 1999
      • Mica Watts, June 1999-July 2000
      • Joshua Nash, June 2000-April 2001
      • Hesham Attaya, September 1999-2003
      • Mohammed Attaya, September 2001-2004
      • Jenny Chua-Tuan, Sept 2001- Sept 2004
    • Summer Accelerated Biomedical Research Program:
      • Keri Shafer, June - August 2000
      • Dustin Powell, June - August 2001
      • Yamina Kebir, June - August 2002
      • Mina Zouggari, June - August 2003
    • TTU Undergraduate Researh Assistants:
      • Tammy Aleman, May 1996 - Sept 1996
      • Renee Arenson, May 1997 - May 1998
      • Eric Dunn, Feb 1998 - Dec 1999
      • Heidi Kunnell, May 1998 - Dec 1999
      • Christina Burnette, Dec 1998 - April 1999
      • Christine Burns, Dec 1998 - May 1999
      • Jothy Thomas, 2002-2003
      • Vazgen Zakaryan, 2005-present
    • UT-Austin:
      • Ravi Gada, June - August 2000
    • MIT:
      • Philip Butler, June - August 2001
    • University of Chicago:
      • Vijay Narendran, June - August 2001
    • High School Students (Clark Scholar's Fellow):
      • Hesham Attaya, June - August 1999
      • Mohamed Attaya, June - August 2001
      • Nisha Wadhani, June - August 2004
    • Texas High School Teachers:
      • Amadu Ba, Ph.D. (Biology), June - Aug 1999
      • Ross Lawson, B.S. (Chemistry), June - Aug 2000
      • Lee Franks, Ph.D. (Biology), June - Aug 2002

 

Updated: February 17, 2014

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