Laboratory Animal Resources Center
The Animal Welfare Act requires that a medical record on all USDA-covered (regulated)
species ("animal") be maintained from the day they arrive at the LARC until the day
they have finished their requirement for the research.
Documentation of Animal Records for IACUC Inspection:
The IACUC requires each investigator to maintain a folder of the current IACUC protocol
(including approval letters), animal records that demonstrate the use of individual
and group animals (i.e. rodents), and laboratory posting of (1) current IBC license
(2) whistleblower notification. The IACUC folder is best maintained as a 3-ring binder
containing (1) a printed copy of the current (and previous, if applicable) IACUC protocol
containing signatures of each protocol member to demonstrate IACUC protocol compliance
(2) the printed IACUC outcome letter showing the protocol with amendments is currently
active and approved (3) animal use records documenting the date animals were used
on study and procedures/treatments given, including humane euthanasia.
Animal Medical Records (Clinical and Surgical):
The research staff is responsible for documenting every research and clinical procedure
in the animal's medical record including daily progress notes. Examples of documentation
required include anesthesia given, medications with dosage and route of administration,
surgery, and humane euthanasia. Any medical treatment given to the animal should
be recorded on the salmon treatment record card (for treatments not related to surgery)
or on the green surgery card (for procedures/treatments/medications relevant to the
Treatments for clinical reasons may be administered by the research staff ONLY after receiving approval by the LARC veterinarian. In addition, regulatory agencies
require that the medical record for each regulated animal (except for USDA-covered
rodents) be retained for at least three years after the animal is no longer in use
in the animal facility.
The LARC provides investigators and their staff with a portable anesthesia machine
to be used for IACUC approved procedures. Some investigators have purchased their
own anesthetic equipment.
It is important to reduce the exposure to waste gases produced in laboratories. Anything
higher than 2 ppm (according to the American 1-h ceiling concentration) is considered
high for stand-alone use of halogenated inhalation anesthetic agents.
We require that investigators maintain weight logs of F/Air canisters. These logs
demonstrate the increase in canister weight with the absorption of isoflurane vapor,
and indicate when the canister must be replaced. Often, for convenience, the weight
log is marked on the side of the canister. The log will be reviewed by the IACUC and
OHS programs during semi-annual inspection to assure the canister is not expired and
Ways to Minimize Exposure to Waste Gases:
If a mask is used, make sure it has a tight seal around the animal's face. If using
F/Air canisters, record the weight before initial use and after each use. After a
50-gram increase, place the canister in a plastic bag before it is discarded. Thoroughly
clean the induction chamber immediately after each use to avoid residual anesthetic
waste release into the environment (which can continue to be released for up to three
hours). Before removing the animal from the induction chamber, close the in-line
valve. The vaporizer must be professionally calibrated at least once per year.
Scavenging System Options:
**BEST: Perform all procedures, including use of the induction chamber, under a fume hood.
No F/Air canister required
**BETTER: Connect a long corrugated hose from your breathing system (and/or induction chamber)
to an exhaust outlet. These outlets can be found by the wall vent. The Physical
Plant division can assess this for you. Waste gases will be taken out through the
vent in to the outside. No F/Air canister required
**GOOD: When using F/Air canisters, the manufacturer recommends replacing the canister after
50 grams of increment in weight relative to the initial weight and/or after 12 hours
of use. Smith and Bolon demonstrated significant release of isoflurane waste into
the environment when canisters continued to be used beyond accumulation of 50 grams
increment. Accordingly, F/Air canisters must be replaced at that point.
Note: The above recommendations are based on: Smith, J.C., & Bolon, B. (2002). Atmospheric
waste isoflurane concentrations using conventional equipment and rat anesthesia protocols.
Contemporary Topics, 41(2).