Luis G. Cuello, Ph.D.
Associate Professor of Cell Physiology & Molecular Biophysics
Texas Tech University Health Sciences Center
3601 4th Street, STOP 6551
Lubbock, Texas 79430
Office: (806) 743-2525
Lab: (806) 743-2527
FAX: (806) 743-1512
I have been trained in the use of several biophysical techniques (electrophysiology, spectroscopy and X-ray crystallography) with the aim to understand at the molecular level the intricate relationship between membrane protein structure and function, with special attention to potassium channels. The application of these techniques in tandem have produced the first structural driven kinetic cycle for a potassium channel, which is a crucial technical achievement that would help us to elucidate the molecular basis of channel related diseases and in the "smart" design of new drugs for therapeutic purposes.
The gating cycle of a K+ channel at atomic resolution
Upper left, Structure of the open and C-type inactivated state at atomic resolution shows a network of water molecules stabilizing KcsA's selectivity filter in the collapsed conformation. Upper right, Inactivating waters behind the selectivity filter are located inside a cavity between to adjacent subunits Inactivation cavity, lower left, Tyrosine 82 in KcsA work as a gatekeeper regulating the flow of water molecules into the inactivation cavity, hence regulating C-type inactivation. Lower right, a small alanine residue at the position 82 allows the free diffusion of water molecules into the inactivation cavity and then accelerating the rate of C-type inactivation.
New Federal Funding
|NIH RO1 (High-resolution crystallographic and functional studies of K+ channel gating )||2018-2022|
|Welch Foundation (Crystallographic and functional studies on the novel role of water molecules in K+
channel C-type inactivation gating)
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Luis Cuello, Ph.D.